Western Blot:Article Title: UBL7 is indispensable for spermiogenesis through protecting critical factors from excessive degradation by proteasomes
Article Snippet: Application Rabbit anti-BMSC UbP (UBL7) Proteintech 11564-1-AP WB 1:1000; IF 1:200 FITC-conjugate PNA Sigma-Aldrich L7381 IF 1:500 Rabbit IgG Isotype Control Invitrogen 02-6102 IF 1:200 Mouse anti-DDX4 / MVH Abcam ab27591 IF 1:500 Mouse anti-α-tubulin Sigma-Aldrich T6199 WB 1:5000; IF 1:500 Rabbit anti-Histone H3 ABclonal A2348 IF 1:500 Rabbit anti-TNP1 Proteintech 17178-1-AP IF 1:200 Rabbit anti-PRM1 Proteintech 15697-1-AP IF 1:200 Mouse anti-Ubiquitin Santa Cruz sc-8017 IF 1:50 Mouse anti-Septin 4 ABclonal A10238 IF 1:200 Mouse anti-Flag Sigma-Aldrich F1804 IP 1:200; IF 1:200 Rabbit anti-DYKDDDDK tag (Flag) Proteintech 20543-1-AP WB 1:3000 Rabbit anti-PGK2 Proteintech 13686-1-AP WB 1:1000; IF 1:100 Rabbit anti-UBE4B Proteintech 18148-1-AP WB 1:1000 Rabbit anti-VCP Proteintech 10736-1-AP WB 1:1000 Rabbit anti-NPLOC4 Proteintech 11638-1-AP WB 1:1000 Mouse anti-Mono- and polyubiquitinylated conjugates (HRP conjugate) Enzo Life sciences ENZABS840HRP0100 WB 1:1000 Rabbit anti-MYC tag Proteintech 16286-1-AP WB 1:3000; IP 1:200 Mouse anti-HA.11 Epitope Tag BioLegend 901501 WB 1:3000; IP 1:200 Rabbit anti-PSMD4 Proteintech 14899-1-AP WB 1:5000 Rabbit anti-PSMB5 Proteintech 19178-1-AP WB 1:1000 Mouse anti-PSMD2 Santa Cruz sc-271775 WB 1:1000 Rabbit anti-SCCPDH Abclonal A2148 WB 1:1000; IF 1:200 Rabbit anti-HK1 Cell Signalling Technology 2024 WB 1:1000; IF 1:200 Rabbit anti-DNPEP Proteintech 15016-1-AP WB 1:5000; IF 1:200 Mouse anti-MAN2C1 Santa Cruz sc-377132 WB 1:1000; IF 1:200 Rabbit anti-SDHA Proteintech 14865-1-AP WB 1:1000 Rabbit anti-PDHB Proteintech 14744-1-AP WB 1:1000 Rabbit anti-SYPL1 Abcam ab184176 WB 1:1000; IF 1:200 Rabbit anti-TCP1 Proteintech 10320-1-AP IF 1:200 Rabbit anti-BSCL2 Abcam ab106793 WB 1:1000 Rabbit anti-GLUT3 (SLC2a3) Proteintech 20403-1-AP WB 1:5000; IF 1:100 Rabbit anti-SPATA20 Proteintech 18373-1-AP WB 1:1000; IF 1:100 Rabbit anti-SPATA6 Proteintech 11849-1-AP WB 1:5000; IF 1:100 Rabbit anti-IFT140 Proteintech 17460-1-AP WB 1:1000 Alexa Fluor® 594 conjugated goat anti-rabbit IgG Invitrogen A-11012 IF 1:500 Alexa Fluor® 488 conjugated donkey antirabbit IgG Invitrogen A-21206 IF 1:500 Alexa Fluor® 594 conjugated goat antimouse IgG Invitrogen A-11001 IF 1:500 Alexa Fluor® 647 conjugated donkey antimouse IgG Invitrogen A-31571 IF 1:500 Alexa Fluor® 488 conjugated donkey antimouse IgG Invitrogen A-21202 IF 1:500 HRP-conjugated goat antirabbit IgG Sigma-Aldrich A6154 WB 1:10000 HRP-conjugated goat antimouse IgG Sigma-Aldrich A4416 WB 1:10000
Control:Article Title: UBL7 is indispensable for spermiogenesis through protecting critical factors from excessive degradation by proteasomes
Article Snippet: Application Rabbit anti-BMSC UbP (UBL7) Proteintech 11564-1-AP WB 1:1000; IF 1:200 FITC-conjugate PNA Sigma-Aldrich L7381 IF 1:500 Rabbit IgG Isotype Control Invitrogen 02-6102 IF 1:200 Mouse anti-DDX4 / MVH Abcam ab27591 IF 1:500 Mouse anti-α-tubulin Sigma-Aldrich T6199 WB 1:5000; IF 1:500 Rabbit anti-Histone H3 ABclonal A2348 IF 1:500 Rabbit anti-TNP1 Proteintech 17178-1-AP IF 1:200 Rabbit anti-PRM1 Proteintech 15697-1-AP IF 1:200 Mouse anti-Ubiquitin Santa Cruz sc-8017 IF 1:50 Mouse anti-Septin 4 ABclonal A10238 IF 1:200 Mouse anti-Flag Sigma-Aldrich F1804 IP 1:200; IF 1:200 Rabbit anti-DYKDDDDK tag (Flag) Proteintech 20543-1-AP WB 1:3000 Rabbit anti-PGK2 Proteintech 13686-1-AP WB 1:1000; IF 1:100 Rabbit anti-UBE4B Proteintech 18148-1-AP WB 1:1000 Rabbit anti-VCP Proteintech 10736-1-AP WB 1:1000 Rabbit anti-NPLOC4 Proteintech 11638-1-AP WB 1:1000 Mouse anti-Mono- and polyubiquitinylated conjugates (HRP conjugate) Enzo Life sciences ENZABS840HRP0100 WB 1:1000 Rabbit anti-MYC tag Proteintech 16286-1-AP WB 1:3000; IP 1:200 Mouse anti-HA.11 Epitope Tag BioLegend 901501 WB 1:3000; IP 1:200 Rabbit anti-PSMD4 Proteintech 14899-1-AP WB 1:5000 Rabbit anti-PSMB5 Proteintech 19178-1-AP WB 1:1000 Mouse anti-PSMD2 Santa Cruz sc-271775 WB 1:1000 Rabbit anti-SCCPDH Abclonal A2148 WB 1:1000; IF 1:200 Rabbit anti-HK1 Cell Signalling Technology 2024 WB 1:1000; IF 1:200 Rabbit anti-DNPEP Proteintech 15016-1-AP WB 1:5000; IF 1:200 Mouse anti-MAN2C1 Santa Cruz sc-377132 WB 1:1000; IF 1:200 Rabbit anti-SDHA Proteintech 14865-1-AP WB 1:1000 Rabbit anti-PDHB Proteintech 14744-1-AP WB 1:1000 Rabbit anti-SYPL1 Abcam ab184176 WB 1:1000; IF 1:200 Rabbit anti-TCP1 Proteintech 10320-1-AP IF 1:200 Rabbit anti-BSCL2 Abcam ab106793 WB 1:1000 Rabbit anti-GLUT3 (SLC2a3) Proteintech 20403-1-AP WB 1:5000; IF 1:100 Rabbit anti-SPATA20 Proteintech 18373-1-AP WB 1:1000; IF 1:100 Rabbit anti-SPATA6 Proteintech 11849-1-AP WB 1:5000; IF 1:100 Rabbit anti-IFT140 Proteintech 17460-1-AP WB 1:1000 Alexa Fluor® 594 conjugated goat anti-rabbit IgG Invitrogen A-11012 IF 1:500 Alexa Fluor® 488 conjugated donkey antirabbit IgG Invitrogen A-21206 IF 1:500 Alexa Fluor® 594 conjugated goat antimouse IgG Invitrogen A-11001 IF 1:500 Alexa Fluor® 647 conjugated donkey antimouse IgG Invitrogen A-31571 IF 1:500 Alexa Fluor® 488 conjugated donkey antimouse IgG Invitrogen A-21202 IF 1:500 HRP-conjugated goat antirabbit IgG Sigma-Aldrich A6154 WB 1:10000 HRP-conjugated goat antimouse IgG Sigma-Aldrich A4416 WB 1:10000
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